Research Paper Volume 14, Issue 21 pp 8645—8660
The deubiquitylase USP7 is a novel cyclin F-interacting protein and regulates cyclin F protein stability
Figure 6. Interaction between USP7 and cyclin F under DNA-damaging condition. HeLa cells were treated with DMSO (control), etoposide (10 μM), or MG132 (10 μM) for 4 h. Endogenous USP7 was immunoprecipitated (IP) with anti-USP7 antibody or an unrelated, anti-p-TAK1 antibody (mock IP) as a loading control. Immunocomplexes were immunoblotted as indicated; β-actin was the loading control. Asterisk denotes non-specific band.