Research Paper Volume 14, Issue 21 pp 8839—8855
lncRNA HHIP-AS1/HHIP modulates osteogenic differentiation of BM-MSCs by regulating Hedgehog signaling pathway
Figure 3. HHIP protein inhibits osteogenic differentiation of BM-MSCs. (A) We constructed HHIP-silenced (si-HHIP) and HHIP-overexpressed (HHIP) BM-MSCs. RUNX2, OCN, ALP and OPN protein levels were detected after 14 days of osteogenic induction of the BM-MSCs. (B) ALP and (C) alizarin red staining after 14 days of osteogenic induction of BM-MSCs. *P < 0.05, **P < 0.01, ***P < 0.001.