Research Paper Volume 14, Issue 23 pp 9599—9616

Figure 9. Downregulation of UBE2C and CDC20 inhibited the proliferation, migration, and immune tolerance in 769-P cells. (A) Representative images and quantitative numbers of migrated cell across the membrane porous over 48 hours were determined. (B) Cell proliferation of 769-P cells with siRNA of CDC20 and UBE2C was detected with CCK8 assay. (C) MTT assay was performed to evaluate the effect of CDC20 and UBE2C knockdown on 769-P cells proliferation after co-cultured with PBMC and THP-1. Mean ± SEM; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Abbreviation: ns: non-significance. All experiments were repeated three times.