Research Paper Volume 14, Issue 23 pp 9679—9698

Figure 3. SKA1 silencing blocks TGF-β1-induced EMT in ccRCC cells. (A) Morphological analysis of 786-O and ACHN cells infected with control (sh-Ctrl) or SKA1 shRNA (sh-SKA1) with or without TGF-β1 treatment. Scale bar = 60 μm. (B) qPCR analysis of E-cadherin (E-cad) and vimentin mRNA levels in 786-O and ACHN cells infected with indicated constructs after TGF-β1 treatment. Mean fold change was calculated from three independent experiments performed in triplicate, with respect to sh-Ctrl-transfected cells artificially set by one. ^*P < 0.05. (C) Western blot analysis of E-cad and vimentin protein levels in 786-O and ACHN cells. One representative experiment of three independent experiments are shown. Numbers indicate fold change in protein levels. (D) Immunofluorescence analysis of E-cad, N-cad and vimentin. Scale bar = 20 μm.