Research Paper Volume 14, Issue 23 pp 9679—9698

Figure 4. SKA1 was physically associated with the SAFB in the nucleus. (A) Pie diagram summarizing the SKA1-bound proteins as revealed by COIP- mass spectrometry. (B and C), Immunoprecipitation and mass spectrometry analysis of cofactors of SKA1. For the coimmunoprecipitation assay, normal IgG served as the negative control. (D) Confocal microscopy scan of immunofluorescence staining shows that SKA1 (green) co-localized with SAFB (red) in the 786-O cells. DAPI was used for nuclear staining. Scale bars = 10 μm. (E) Kaplan-Meier analysis of the overall survival in ccRCC patients from TCGA-KIRC (N = 242) according to the concurrent expression of SKA1 and SAFB.