Research Paper Volume 14, Issue 23 pp 9758—9772
A pan-cancer analysis of ring finger protein 135 and its relationship to triple-negative breast cancer proliferation and metastasis
Figure 6. Relationship between RNF135 and viability of MDA-MB-231 cells. (A) qRT-PCR assay shows the transcriptional levels of the RNF135 gene with GAPDH used as the loading control in MDA-MB-231 and MDA-MB-231-luc cells. (B) Effect of sh-RNF135 on the proliferation of MDA-MB-231 cells was detected by CCK-8 assays. (C) The cell cycle distribution of MDA-MB-231 cells were measured using propidium iodide staining and flow cytometry analyses. Data are presented as the mean ± SD for three independent experiments (*P < 0.05) (Original magnification: 100×).