Figure 7. Relationship between RNF135 and migration ability of MDA-MB-231 cells. (A) Migration of MDA-MB-231 cells was detected via wound healing assay. (B) Changs in cellular morphology of MDA-MB-231 cells were investigated via 3D culture. (C) Bioluminescent imaging of mice harboring metastases after tail vein injection of MDA-MB-231-luc cells at week 5. Data are presented as the mean ± SD for three independent experiments (*P < 0.05) (Original magnification: 100×).