Research Paper Volume 15, Issue 1 pp 70—91

Figure 1. Smyd2 is upregulated in vascular aging both in vivo and in vitro. (A, B) The in vivo aging model was established by Ang II infusion in mice for 28 days and harvested the aortas. The protein expressions of Smyd2, VCAM-1, and p21 were detected by immunoblots (A). The immunohistochemistry assay was performed with Smyd2 antibody. Scale bars, 50 μm (B). (C–E) Ang II (100 nM, 48 h)-induced RAECs was used as the in vitro aging model. Smyd2 and the senescence-related phenotype markers were increased in a dose-dependent manner in Ang II-induced RAECs (C). The protein expression of Smyd2 was increased in a time-dependent manner in Ang II-induced RAECs (D). RT-qPCR analysis of Smyd2 and Cdkn1a genes in Ang II-induced RAECs (E). Data are presented as the mean ± SEMs, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, each acquired from three individual experiments (n = 3).