Research Paper Volume 15, Issue 1 pp 70—91

Figure 2. Knockdown of Smyd2 attenuates Ang II-induced RAECs senescence. Smyd2 was knocked down using si Smyd2 in Ang II-induced RAECs, si CTL was used as the negative control. (A, B) The SA-β-gal staining and EdU incorporation assay. Representative staining images are shown in the figure left, and the statistical analysis of positive cells is shown on the right. Scale bars, 200 μm. (C) Western blot analysis of Smyd2 and the senescence markers (p53, p21 and p16), the proinflammatory mediators (iNOS, VCAM-1, COX-2 and IL-6) and the DNA damage marker (p-ATM) protein. Representative western blot images are shown in the figure above, and the statistical analysis of relative protein expression is shown below. GAPDH was used as the loading control. (D) The mRNA expression of Il6 gene by RT-qPCR analysis. (E, F) Immunofluorescence double staining of Smyd2 and the senescence markers (p53 and p16). Scale bars, 100 μm. Data are presented as the mean ± SEMs, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, each acquired from three individual experiments (n = 3).