Research Paper Volume 15, Issue 1 pp 70—91

Figure 4. Overexpression of Smyd2 alone promotes the vascular endothelial senescence. Smyd2 was overexpressed (Smyd2 OE) in RAECs by transfecting with lentiviruses. (A, B) SA-β-gal staining (A) and EdU incorporation (B) assays in RAECs. Representative staining images are shown in the figure left, and the statistical analysis of positive cells is shown in the figure right. (C) Immunoblots of the senescence-associated markers (p53, p21, p16 and iNOS) proteins were detected by western blot assay, and the statistical analysis of relative protein expression is shown below. GAPDH was used as the loading control. (D, E) Immunofluorescence double staining of Smyd2 and p53 or p16 protein in RAECs transduced with lentiviruses expressing Smyd2. Scale bars, 100 μm. Data are presented as the mean ± SEMs, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, each acquired from three individual experiments (n = 3).