Research Paper Volume 15, Issue 1 pp 70—91

Figure 6. Smyd2 upregulation activated distant enhancers adjacent to Cdkn2a (p16) and Cdkn1a (p21) genes. (A) Western blot analysis of the Smyd2 and H3K4me1 level in Ang II-treated RAECs by the time-dependent manner. (B) The H3K4me1 level was detected after Smyd2 knockdown in Ang II-treated RAECs. (C) The ChIP-seq visualization data with H3K27Ac, H3K4me1 as well as H3K4me3 antibodies of Cdkn2a (p16) and Cdkn1a (p21) genes in Ang II-treated or control RAECs, as shown in Integrative Genomics Viewer (IGV). (D) The potential enhancer regions of Cdkn2a and Cdkn1a genes, namely peak 1–4, were evaluated by ChIP-PCR in Ang II-treated RAECs after Smyd2 knockdown. Data are presented as the mean ± SEMs, ^**p < 0.01, ^***p < 0.001, each acquired from three individual experiments (n = 3).