Research Paper Volume 15, Issue 1 pp 70—91

Figure 7. Smyd2 is associated with other vascular aging-related diseases. (A) The protein expressions of Smyd2, the senescence marker (p21) and the proinflammatory mediators (VCAM-1, COX-2 and IL-6) were examined in a later passage of RAECs by Western blot. GAPDH was used as the loading control. (B) Western blot analysis of Smyd2 and the senescence markers (p21, VCAM-1 and IL-6) in arteries of two-kidney one-clip renovascular hypertensive (2K1C) mice. The statistical analysis of relative protein expression is shown in the figure below. (C) Western blot analysis of Smyd2 in arteries of db/db mice and its control db/m mice. (D) The mRNA levels of Smyd2 and the senescence marker genes (Ptgs2, Cdkn1a and Trp53) in arteries of db/db mice and its control db/m mice. (E) The expression of Smyd2 in mice atherosclerotic artery samples was evaluated by western blot. (F) The protein level of Smyd2 in thoracic aorta in healthy (control) and atherosclerotic (AS) patients evaluated by western blot. Data are presented as the mean ± SEMs, n = 3 or 6. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.