Research Paper Volume 15, Issue 1 pp 246—260
Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes
Figure 4. MitoQ elevates the mitochondrial oxygen consumption of human granulosa cells. (A) Analysis of cellular mitochondrial function by oxygen consumption rate (OCR) through Seahorse Bioscience Analyzer. All OCR values for different stages of basal respiration (B), maximal respiration (C), ATP production (D), reserved respiration (E), proton leakage (F), and non-mitochondrial respiration (G) were analyzed, and control, MitoQ, ROS, and ROS/MitoQ groups were analyzed. Oligomycin (1 μM), FCCP (1 μM), antimycin (0.5 μM), and rotenone (0.5 μM). *p < 0.05, **p < 0.01 and ***p < 0.001, as compared with control. #p < 0.05, ##p < 0.01 and ###p < 0.001 compared to ROS only.