Figure 4. Effects of immunofluorescence on microglia in mice. (A) Effects of luteolin on microglia in different doses were determined by immunofluorescence. (B) Determination of microglia in the hippocampal area of mice after EX-527. (C) Effect of EX-527 on microglia activation. (D) Relative fluorescence intensity of Nlrp3. Data were analyzed by mean ± SEM. Compared with blank group, ##P < 0.01; Comparison with model group *P < 0.05, **P < 0.01, n = 9.