Figure 4. Increases of osteogenesis and ERK1/2 activation in GD1a-treated Lmna dysfunction MSCs. … dysfunction MSCs. (F) Lamin A/C was knocked down in mouse MSCs using siRNA. (G) Primary MSCs with LmnaDhe/+ mutation were isolated from LmnaDhe/+ mice. (H) Phosphorylation of ERK1/2 in Lmna dysfunction MSCs treated with GD1a. (I)…. Phosphorylation of ERK1/2 was determined by western blotting with anti-p-ERK1/2. ERK1/2 was used as a loading control. Values represent mean ± SD; &&&p < 0.001 indicates a significant difference from the normal MSCs; ***p < 0.001 indicates a significant difference from the LmnaDhe/+ mutant MSCs; ###p < 0.001 indicates a significant difference from the Lamin A/C KD MSCs. $$$p < 0.001 indicates a significant difference from the U0126-treated MSCs.