Research Paper Volume 15, Issue 3 pp 630—649

Figure 1. Immune-mediated platelet depletion in APP-PS1 mice. (A) APP-PS1 mice were intraperitoneally injected with an anti-CD42b antibody (αCD42b) to induce thrombocytopenia. The injection of non-immune rat immunoglobulins (IgG) was used as a control. (B) Experimental setup. (C) Platelet depletion efficacy was monitored by whole-blood flow cytometry using an allophycocyanin (APC)-labelled anti-mouse CD41 antibody to label platelets (PLTs). Representative flow cytometry plots and gate strategy. (D) Anti-CD42b antibody administration effectively reduced blood platelet counts. (E) Brain tissue immunolabelled for CD41 (green) was used to quantify platelets in the hippocampus. DAPI (blue) was used as nucleus staining. (F) Platelets were significantly reduced in the hippocampus of platelet-depleted mice. Data are shown as mean ± SEM. Statistical analysis was performed by two-way ANOVA with Holm-Šídák’s multiple comparisons test (D: n = 8–9/treatment) and unpaired Student’s t test (F: n = 8–9/treatment; “full forms” represent females and “empty forms” males). ^****p < 0.0001; ^**p < 0.01. Scale bar: 50 μm. (A, B) Created with Biorender. Abbreviations: PLTs: platelets; RBCs: red blood cells; Coinc. Evts: Coincident platelet and red blood cell events.