Research Paper Volume 15, Issue 3 pp 791—809

Figure 1. Classification of hair follicle mesenchymal stem cells (HF-MSCs) and HFMSC-derived exosomes (HF-MSC-Exo). (A) Morphological observation of MSCs (200×). (B) MSCs surface marker molecules identified by flow cytometry. HF-MSCs were positive for CD90, CD105, CD73, CD44 and negative for CD34, CD45 and CD31. (C) Cell lineage-induced chondrogenic, osteogenic, and adipogenic differentiation were evaluated by toluidine blue staining, alizarin red staining, and Oil-Red-O staining. Scale bar=100 μm. (D) Micrographs of transmission electron microscopy of purified HF-MSC-Exo, showing a spheroid shape. Scale bar=50 nm. (E) The size distribution of the HF-MSC-s was assessed utilizing dynamic light scattering. (F) Western blotting results indicated the positive expression of Alix, CD63, and Tsg101 protein in HF-MSC-Exo.