Research Paper Volume 15, Issue 3 pp 791—809

Figure 4. HF-MSC-Exo inhibited HG-induced pyroptosis of HaCaT cells and promoted their proliferation. (A) HaCaT cells were treated with HG and then treated with HFMSC-dp-Exo or HF-MSC-Exo for 24-72 h. The CCK-8 assay findings illustrated that the cell viability was higher in the presence of HF-MSC-Exo than in the control and also higher than the HF-MSC-dp-Exo cohort. (B, C) The flow cytometric assay results showed that the HF-MSC-Exo can inhibit HG-induced apoptosis of HaCaT cells. (D, E) The flow cytometric assay results showed that the HF-MSC-Exo can reduce HG-induced fluorescence intensity in HaCaT cells. Western blotting (F) for caspase-1 (G), IL-1β (H), IL-18 (I), and NLRP3 (J) in HaCaT cells. Compared to control: *P<0.05 and **P<0.01 representing three separate experiments (means ± SD).