Research Paper Volume 15, Issue 3 pp 791—809

Figure 5. HF-MSC-Exo can be uptaken by HaCaT cells and promote the migration of HG-treated HaCaT cells. (A) HF-MSC-Exo was labeled with PKH26, a lipid membrane-intercalating dye. The labeled exosomes were introduced to the culture medium of HaCaT cells. After 24 hours, HaCaT cells were fixed, counterstained with Hoechst 33342, and analyzed by fluorescent microscopy. The images showed that the labeled HF-MSC-Exo entered into the cytoplasm of HaCaT cells. Scale bar=200 μm. HaCaT cells were treated with HG and subsequently cultured in the presence of HF-MSC-Exo or HF-MSC-dp-Exo for 24 hours. The control cohort was the normal HaCaT cells. (B, C) HaCaT cells skin wound healing and cell migration experiment after treated with different culture medium for 24hours. (D, E) Quantified the proportion of wounded area closure and cell migration rate. (n=3); **P<0.01 Control vs HG; ^##P<0.01, dp+Exo vs HG; ^&&P<0.01, Exo vs HG). Scale bar = 200 μm. All results are representative of three independent experiments (means ± SD).