Research Paper Volume 15, Issue 3 pp 791—809

Figure 7. Exosomes overexpressing lncRNA H19 affected HaCaT cell proliferation, apoptosis, migration, and pyroptosis. (A) HaCaT cells were treated with HG and subsequently cultured in the presence of OE-H19-exosomes, sh-H19-exosomes, and NC-exosomes. The CCK-8 assay findings illustrated that cell viability was higher in the OE-H19-exosomes cohort than in the sh-H19-exosomes cohort. (B, C) HaCaT apoptosis identified by TUNEL assay (200×). *P<0.05 and **P<0.01versus the 5.5 mM cohort. Data are articulated as mean±SD (n=6), and the experiment was redone separately three times. (D, E) HaCaT cells were treated with OE-H19-exosomes, sh-H19-exosomes, and NC-Exo were exposed to a wound-healing assay and transwell migration assay for 12 hours. Scale bar =200μm. (F, G) Statistic the wound area closure and the number cell of per filed. Scale bar=200 μm. All results are representative of three separate experiments (means ± SD). Western blotting (H) for caspase-1 (I), IL-1β (J), IL-18 (K), and NLRP3 (L) expression in HaCaT cells. **P<0.01 OE-19 vs NC; ^##P<0.01, sh-19 vs NC; ^&&P<0.01, OE-19 vs sh-19), representative of three independent experiments (means ± SD).