Research Paper Volume 15, Issue 6 pp 1931—1943

Figure 2. Exosomes derived from cerebral I/R rats exhibit miR-370-3p upregulation. (A) The levels of CD63, TSG101, and α-Tubulin were tested by western blotting. (B) Cerebral MVs-derived exosomes were analyzed via electron microscopy (scale bar, 50 nm). (C) Relative miR-370-3p expression in exosomes derived from MVs were tested by qPCR. (D) After treatment with 2 mg/ml RNase alone or combined with 0.1% Triton X-100, miR-370-3p level was analysed using qPCR in the culture medium of MVs. (E) miR-370-3p expression was assessed by fluorescence in situ hybridization (FISH) in cerebral I/R rats and healthy rats. (F) miR-370-3p expression levels in bEND.3, were assessed via qPCR. (G) After treatment with 2 mg/ml RNase alone or combined with .1% Triton X-100, miR-370-3p level was analysed using qPCR in the culture medium of bEND.3. (H) Quantitative analysis indicated that relative fluorescence intensity in bEND.3. ** p<0.01, *** p<0.005, **** p<0.001.