Research Paper Volume 15, Issue 6 pp 1931—1943
Exosomal miR-370-3p increases the permeability of blood-brain barrier in ischemia/reperfusion stroke of brain by targeting MPK1
Figure 3. GW4968 and Rab27a silencing down-regulate miR-370-3p in cerebral MVs. (A) Following co-culture with exosomes treated with GW4869, miR-370-3p was analyzed in bEND.3 cells. (B) After Rab27a silencing, miR-370-3p levels in bEND.3 cells were tested by qPCR. (C, D). miR-370-3p levels in bEND.3 cells were measured via qPCR following miR-370-3p mimic or inhibitor treatment. (E, F) Cerebral MVs from I/R rats were collected and then transfected with miR-370-3p mimic or inhibitor. miR-370-3p expression in exosomes from these cells then being assessed via qPCR. ns, not significant, ** p<0.01, *** p<0.005, **** p<0.001.