Research Paper Volume 15, Issue 6 pp 2097—2114

Figure 2. ADAMTS1 promotes migration, invasion, and EMT of A549 cells. ADAMTS1 overexpression plasmids or si-ADAMTS1 were transfected into A549 cells. (A) Wound-healing assay was used for detecting the migration of A549 cells transfected with ADAMTS1 overexpression plasmids. (B) Transwell examined the migration and invasion of A549 cells (scale bar=50 μm). (C) Protein expressions of E-cadherin, N-cadherin, and Vimentin in A549 cells were detected using Western blot. NC=negative control. (D) The wound-healing assay showed the migration of A549 cells after the knockdown of ADATMS1. (E) Transwell assays showed the migration and invasion of A549 cells after the knockdown of ADATMS1 (scale bar=50 μm). (F) Protein expressions of E-cadherin, N-cadherin, and Vimentin in A549 cells were detected using Western blot. (G) EdU staining was used for detecting cell proliferation. Scale bar=100 μm. **P<0.01, ***P<0.001 compared with NC or si-NC group. N=3.