Research Paper Volume 15, Issue 6 pp 2097—2114

Figure 7. Effects of ADAMTS1 and si-TGF-β on proliferation, cycle, migration, invasion, and EMT of the NSCLC cell line A549 and H226. (A) qPCR showed that TGF-β was significantly reduced after transfecting siTGF-β into A549 cells. (B) The wound-healing assay showed migration of A549 cells co-transfected with ADAMTS1 and siTGF-β was significantly lower than that of cells transfected with ADAMTS1 (C) Transwell assay was used to detect migration and invasion of A549 cells (scale bar=50 μm). (D) Protein expressions of E-cadherin, N-cadherin, and Vimentin in A549 cells were detected using Western blot. (E) qPCR showed that TGF-β was significantly reduced after transfecting siTGF-β into H226 cells. (F) The wound-healing assay showed migration of H226 cells co-transfected with ADAMTS1 and siTGF-β was significantly lower than that of cells transfected with ADAMTS1. (G) Transwell assay was used to detect migration and invasion of H226 cells (scale bar=50 μm). (H) Protein expressions of E-cadherin, N-cadherin, and Vimentin in H226 cells were detected using Western blot. NS P>0.05, **P<0.01, ***P<0.001. N=3.