Research Paper Volume 15, Issue 6 pp 1808—1832
Cellular senescence and disrupted proteostasis induced by myotube atrophy are prevented with low-dose metformin and leucine cocktail
Figure 1. MET+LEU increased myotube area during differentiation. (A) Experimental schematic of treatments during differentiation. (B–E) Representative images of control, MET, LEU, and MET+LEU. (F) Myotube area after 4 days of differentiation and 0.1, 25, 100 μM MET, (G) 0.5, 125, and 500 μM LEU, (H) 0.1 + 0.5, 25 + 125, 100 + 500 μM MET+LEU. All data are represented as fold change from control. **p < 0.01, *p < 0.05. N = 3–12/group. Scale bar represents 200 μm.