Research Paper Volume 15, Issue 6 pp 1808—1832
Cellular senescence and disrupted proteostasis induced by myotube atrophy are prevented with low-dose metformin and leucine cocktail
Figure 2. SD caused myotube area loss that is prevented with higher concentrations of MET, LEU, and MET+LEU. (A) Experimental schematic of treatments during SD. (B–C) Representative images of 2% HS and SD. (D) Myotube area with 2% HS or SD for 4 days. (E) Myogenic index (the percentage of total nuclei within myotubes) with 2% HS or SD for 4 days. Dose-response experiment with (F) MET, (G) LEU, and (H) MET+LEU during 4 days of SD. All data are represented as fold change from 2% HS. *p < 0.05 vs. SD, #p < 0.05 vs. 2% HS. N = 3–16/group. Scale bar represents 200 μm.