Research Paper Volume 15, Issue 6 pp 1808—1832

Figure 5. MET+LEU reduced inflammatory transcriptional profile and markers of cellular senescence. (A) Inflammation-related genes from RNA-sequencing in 2% HS, SD, and MET+LEU as fold change from 2% HS. (B) Venn diagram of how 378 genes altered with SD and reversed with MET+LEU was determined. (C) Protein-protein interaction map displaying transcripts reversed with MET+LEU was in the interconnected signaling networks of TNF and MAPK pathways. (D) Pathways altered with MET+LEU vs. SD related to cellular senescence, numbers are representative of NES. (E) Heatmap of gene expression represented as a fold change from 2% HS of genes within the cellular senescence-related pathways. (F) TRRUST enrichment analysis of predicted regulators of transcriptional changes with MET+LEU vs. SD in reversed genes. (G) Cellular senescent-related genes from RNA-sequencing in 2% HS, SD, and MET+LEU as fold change from 2% HS. (H) SA-β-galactosidase area analysis in 2% HS, SD, and MET+LEU. (I–K) Representative images of SA-β-galactosidase staining. (L) Myotube area during 4 days SD, MET+LEU or 62.5 nM dasatinib + 12.5 μM quercetin (D+Q). (M–P) Representative images for analyzing myotube area. All data are after 4 days of SD. ^****p < 0.0001, ^***p < 0.001, ^**p < 0.01, ^*p < 0.05. N = 6/group for (A–G), N = 7/group for (H–K). N = 4/group for (L–P). Scale bar represents 200 μm.