Research Paper Volume 15, Issue 6 pp 1808—1832
Cellular senescence and disrupted proteostasis induced by myotube atrophy are prevented with low-dose metformin and leucine cocktail
Figure 6. MET+LEU prevented aged myofiber atrophy and improved myonuclei fusion in primary human myotubes from an aged donor. (A) Mean myofiber diameter in single isolated myofibers from aged mice with 0, 2, 3, and 4 days of either 2% HS, SD, or MET+LEU during SD. (B–D) Representative images of isolated myofibers with different treatments. (E) Myogenic index in primary myotubes from an aged donor following 8-days differentiation then 8-days of 2% HS, SD, or MET+LEU during SD represented as fold change from 2% HS. (F–H) Representative images of primary myotubes, yellow label indicates nuclei fused into myotubes. **p < 0.01, N = 7–10/group for (A–D) and N = 15–16/group for (E–H). Scale bar represents 200 μm.