Research Paper Volume 15, Issue 6 pp 2308—2320

Figure 1. Schedule of experiment design and the results of treadmill test and body weight. (A) Arrangement of experimental design, after seven days of acclimatizing to the environment and weighing, the mice in the treatment group received 28 days of intragastric administration, during which they were weighed once a week. The treadmill test was carried out from d20 to d28, starting with a three-day pre-training period on d20, followed by a six-day formal experiment. (B) An illustration of the treadmill test, where the animal was moving at a speed of 18m/min on the track and was considered to have reached a state of exhaustion when it continued to move in the posterior third of the platform and received frequent electric shocks. (C) The results of body weight. On day -7(caffeine: p=0.432, curcumin: p=0.602), On day 1(caffeine: p=0.984, curcumin: p=0.996), On day 7(caffeine: p=0.804, curcumin: p=0.910), On day 14(caffeine: p=0.902, curcumin: p=0.892), On day 21(caffeine: p>0.999, curcumin: p=0.597), On day 28(caffeine: p=0.082, curcumin: p=0.277) compared to the control group. No significant differences could be observed in the time×column factor [F(10, 285) = 1.593, p=0.108] and the column factor [F(2, 57) = 0.237, p=0.790], but the time factor [F(2.290, 130.5) = 712.0, p<0.0001] did have a significant effect on the change in body weight of the mice. Data are shown as mean ± SEM. (D) Exhaustion time of the mice in the curcumin group and caffeine group (control group: n=22, dosed groups: n=19).