Research Paper Volume 15, Issue 7 pp 2373—2394

Figure 1. Induction of senescence in the EJ models. (A) Representative images of the SA-β-Gal staining of EJp53, EJp21 and EJp16 uninduced (Control, proliferating) or 6 days after tet removal to induce the expression of p53, p21 or p16, respectively (Senescent). Black arrows point to examples of senescent cells. The graph on the right is a quantitation of SA-β-Gal positive cells of three independent experiments. **, P < 0.01; ***, P < 0.001. (B) Cell counting of the same cell lines over a period of six days since tet removal. Three independent experiments were performed in duplicates and the averages and standard deviation (SD) were plotted in the graphs. (C) Representative Western blots of lysates of the same cell lines showing expression of the proteins induced by tet removal in each cell line. Actin was used as a loading control.