Research Paper Volume 15, Issue 6 pp 2347—2357

Figure 3. Knockdown of ALDH1A3 enhanced radiosensitivity in GBM cell lines. (A) The mRNA levels of ALDH1A3 decreased when GBM cells were transfected with siRNA. (B) The growth rate of GBM cells was reduced by transfection with si-ALDH1A3. (C) The colony formation assay showed a dramatic decrease in the survival rate of si-ALDH1A3 transfected GBM cells when exposed to different doses of irradiation (0 Gy, 2 Gy, 4 Gy and 6 Gy). (D) A significant decrease in cell colonies treated with si-ALDH1A3 and 6 Gy irradiation was observed compared with the control group in GBM cell lines. (E) The apoptosis rates of GBM cells increased significantly by silencing ALDH1A3 and exposure to 6 Gy irradiation. NC represents negative control. ^*p < 0.05, ^**p < 0.01.