Research Paper Volume 15, Issue 6 pp 2347—2357

Figure 4. miR-320b is an upstream miRNA that interacts with ALDH1A3 in GBM cell lines. (A) The Venn diagram was used to illustrate the miRNAs that were common in the TargetScan, miRWalk, and ENCOR1 datasets. (B) Among the five miRNAs tested on GBM cell lines, miR-320b had the strongest impact on reducing ALDH1A3 levels. (C) Overexpression of miR-320b caused a decrease in the amount of ALDH1A3 protein in GBM cell lines. (D) The luciferase reporter constructs, ALDH1A3 3′UTR-WT and ALDH1A3 3′UTR-MUT, were created using the seed sequence of miR-320b that matched the 3′UTR of ALDH1A3 mRNA. (E) Luciferase activities were notably deceased after co-transfection of miR-320b mimics and ALDH1A3 WT. WT represents wild type. MUT represents mutation. NC represents negative control. ^*p < 0.05, ^**p < 0.01.