Figure 2. Cellular senescence in human salivary gland tissue. (A) β-Galactosidase assay, TUNEL assay, and the expression of 8-OHdG. (B) Quantitation of β-galactosidase positive areas. (C) Quantitative analysis of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive areas. (D) Quantitative analysis of 8-OHdG (8-hydroxy-2' -deoxyguanosine) positive areas. Results are presented as the mean ± SD and T-test was performed for statistical analysis ****p < 0.0001, ***p < 0.001, **p < 0.01.