Figure 5. Cellular senescence and the expressions of aging-related functional proteins in primary cultured salivary gland (SG) cells. (A) β-Galactosidase stained adult and aged primary cultured SG cells. (B) The proliferation rate of adult and aged human SG cells. (C–E) The mRNA expressions of ADIPOQ, amylase, and AQP5 in adult and aged human parotid and submandibular gland tissues. (F–I) The protein expressions of ADIPOQ, amylase, and AQP5 in adult and aged human parotid and submandibular gland tissues. Results are presented as the mean ± SD and T-test was performed for statistical analysis, ***p < 0.001, **p < 0.01, *p < 0.05.