Research Paper Volume 15, Issue 6 pp 1840—1858
Adiponectin is associated with inflammaging and age-related salivary gland lipid accumulation
Figure 6. Silencing of ADIPOQ reduced cellular senescence and enhanced the proliferation and expression of salivary functional proteins. (A) β-Galactosidase assays of scrambled-siRNA and ADIPOQ-siRNA transfected primary cultured SG cells. (B) Proliferations of ADIPOQ-siRNA and scrambled siRNA treated SG cells. (C–E) The mRNA expressions of ADIPOQ, amylase, and AQP5 in ADIPOQ-siRNA and scramble-siRNA treated SG cells. (F–I) The protein levels of ADIPOQ, amylase and AQP5 in ADIPOQ-siRNA and scramble-siRNA treated SG cells. Results are presented as the mean ± SD and T-test was performed for statistical analysis, ****p < 0.0001, ***p < 0.001, *p < 0.05.