Research Paper Volume 15, Issue 7 pp 2395—2417

p21 facilitates chronic lung inflammation via epithelial and endothelial cells


Figure 4. p21-/- protects lung airways from chronic inflammatory responses triggered by chronic LPS exposure. WT and p21-/- mice were exposed to either PBS or aerosolized LPS (0.5 mg/ml), 3 times a week for 10 weeks. (AF) At 48 hours following the last LPS exposure BAL fluid was collected and flow cytometry was used to determine: (A) numbers of neutrophils (CD45+/Ly6G+/CD11b+), (B) numbers of alveolar macrophages (CD45+/CD11c+/SiglecF+), (C) numbers of B cells (CD45+/B220+), (D) numbers of CD3+ T cells (CD45+/CD3+), (E) numbers of CD4+ T cells (CD45+/CD3+/CD4+), and (F) numbers of CD8+ T cells (CD45+/CD3+/CD8+). Data information: Data were analyzed using one-way ANOVA. ***P<0.0005. Data represent mean ±SEM (AF, n=8-11 independent repeats).