Research Paper Volume 15, Issue 7 pp 2689—2704

Long noncoding RNA POU6F2-AS2 contributes to the aggressiveness of nonsmall-cell lung cancer via microRNA-125b-5p-mediated E2F3 upregulation


Figure 6. E2F3, a target of miR-125b-5p, is controlled by POU6F2-AS2 in NSCLC cells. (A) miR-125b-5p possessed binding sequences within E2F3. (B) Luciferase activity induced by WT-E2F3 or MUT-E2F3 was examined in NSCLC after miR-125b-5p upregulation. **P < 0.001 vs. miR-NC group. (C and D) MTDH mRNA and protein levels in miR-125b-5p overexpressed-NSCLC cells. **P < 0.001 vs. miR-NC group. (E and F) H460 cells were transfected with si-NC, si-POU6F2-AS2, si-POU6F2-AS2+NC inhibitor, or si-POU6F2-AS2+miR-125b-5p inhibitor. SK-MES-1 cells were transfected with pcDNA3.1, pc-POU6F2-AS2, pc-POU6F2-AS2+miR-NC, or pc-POU6F2-AS2+miR-125b-5p mimic. After transfection, the quantification of E2F3 levels was conducted. **P < 0.001 vs. si-NC and pcDNA3.1 groups. ##P < 0.001 vs. si-POU6F2-AS2+NC inhibitor and pc-POU6F2-AS2+miR-NC groups.