Research Paper Volume 15, Issue 7 pp 2689—2704

Long noncoding RNA POU6F2-AS2 contributes to the aggressiveness of nonsmall-cell lung cancer via microRNA-125b-5p-mediated E2F3 upregulation


Figure 7. miR-125b-5p inhibition or E2F3 upregulation reverses the repressing effects of si-POU6F2-AS2 in NSCLC cells. (A) The interference efficiency of miR-125b-5p inhibitor in H460 cells. **P < 0.001 vs. NC inhibitor group. (B) The transfection efficiency of pc-E2F3 in H460 cells by western blotting. **P < 0.001 vs. pcDNA3.1 group. (CE) H460 cells were transfected with si-NC, si-POU6F2-AS2, si-POU6F2-AS2+NC inhibitor, si-POU6F2-AS2+miR-125b-5p inhibitor, si-POU6F2-AS2+pcDNA3.1, or si-POU6F2-AS2+pc-E2F3. The capacities of proliferative and colony formation were, respectively, measured by CCK-8 and colony formation assays. (F) Transwell migration and invasion assays of the motility of H460 cells treated as abovementioned. **P < 0.001 vs. si-NC group; ##P < 0.001 vs. si-POU6F2-AS2+NC inhibitor group; &&P < 0.001 vs. si-POU6F2-AS2+pc-E2F3 group.