Research Paper Volume 15, Issue 7 pp 2689—2704

Long noncoding RNA POU6F2-AS2 contributes to the aggressiveness of nonsmall-cell lung cancer via microRNA-125b-5p-mediated E2F3 upregulation


Figure 8. miR-125b-5p overexpression or E2F3 knockdown counteracts the actions triggered by pc-POU6F2-AS2 in NSCLC cells. (A) E2F3 level in SK-MES-1 cells after pc-E2F3 or pcDNA3.1 transfection. **P < 0.001 vs. si-NC group. (B and C) POU6F2-AS2-overexpressed SK-MES-1 cells were treated with miR-125b-5p mimic or pc-E2F3. The assessment of cell proliferation and colony formation was implemented applying CCK-8 and colony formation assays, respectively. *P < 0.01 and **P < 0.001 vs. pcDNA3.1 group. ##P < 0.001 vs. pc-POU6F2-AS2+miR-NC and pc-POU6F2-AS2+si-NC groups. (D) Transwell migration and invasion assays were operated to measure cell motility in abovementioned cells. **P < 0.001 vs. si-NC group. ##P < 0.001 vs. pc-POU6F2-AS2+miR-NC group. &&P < 0.001 vs. pc-POU6F2-AS2+si-NC group.