Research Paper Volume 15, Issue 7 pp 2705—2720

Figure 1. Berberine promoted the phenotype of microglia/macrophage transformed into anti-inflammation and ameliorated ICH-induced brain injury and improves neurological function. (A, B) Immunostaining for CD16⁺Iba-1⁺/Iba-1⁺ in ICH + Vehicle and ICH + Ber group after ICH day 3. (n = 5 per group). (C, D) Immunostaining for Arg-1⁺Iba-1⁺/Iba-1⁺ in ICH + Vehicle and ICH + Ber group after ICH day 3. (n = 5 per group). (E–G) The levels of mRNA transcription of IL-1β, TNF-α, and CD16 were examined by qRT-PCR in sham, ICH + Vehicle, and ICH + Ber groups after ICH day 3. (n = 6 per group). (H, I) Relative mRNA expression of Arg-1 and CD206 in sham, ICH + Vehicle, and ICH + Ber groups after ICH day 3. (n = 6 per group). (J–L) The percentage of cylinder test, forelimb test, and the score of wire hanging test in ICH + Vehicle and ICH + Ber group. (n = 10 per group). Data are expressed as the mean ± SEM. ^#P < 0.05. ^##P < 0.01. ^###P < 0.001 sham vs. ICH + Vehicle group. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001 ICH + Vehicle group vs. ICH + Ber group. Scale bar = 100 μm.