Research Paper Volume 15, Issue 7 pp 2797—2811

Figure 4. Dex protected neuronal cells from apoptosis induced by ischemia/reperfusion through regulating YBX1 expression. (A) Venn diagram shows the numbers of overlapping genes among the predicted genes of miR-337-3p using the online algorithms including miRDB, TargetScan and microT. (B) Schematic diagram shows the matching base pairs between miR-337-3p and the wild type (WT) or mutated (MUT) 3′-UTR of Ybx1 mRNA. (C) The luciferase activity of the reporter vectors was detected in Neuro-2a cells at 48 hours after co-transfection of the plasmid expressing wild type or mutant 3′-UTR of Ybx1 mRNA together with the NC mimic or the miR-337-3p mimic. (D, E) Neuro-2a cells were transfected with control miRNA inhibitor or miR-337-3p inhibitor, and the expression of miR-337-3p (D) and protein level of Ybx1 (E) were quantitated at 48 hours after transfection. (F) Neuro-2a cells were transfected with control miRNA mimics or miR-337-3p mimics, and left untreated or received OGD/R. The protein level of Ybx1 was quantitated. (G) Effects of miR-337-3p inhibitor transfection and Dex treatment, alone or in combination, in OGD/R-treated Neuro-2a cells on the expression of Ybx1 protein. (H) Confirmation of Ybx1 overexpression in Neuro-2a cells at 48 hours after transfection of Ybx1-overexpression (OE) plasmid. (I, J) Control cells or Ybx1-OE cells were left untreated or received OGD/R. Cell proliferation (I) and apoptosis (J) in the indicated groups were measured. ^*P < 0.05, compared with the Control group or between the indicated groups.