Research Paper Volume 8, Issue 12 pp 3419—3429

Figure 1. Western blotting of PrP captured by QSOX. (A) PrP eluted from the QSOX-conjugated beads was detected by Western blotting with the anti-PrP antibody 3F4. The g5p-beads and OCD4-beads were used as controls while the PDI beads served as a negative control. Like g5p and OCD4, QSOX captures PrP only from CJD but not from non-CJD control brain homogenate. (B) PrP was captured by the QSOX beads from brain homogenates of two sCJD cases (sCJD1 and sCJD2), two cases with different genetic prion diseases (fCJD1 and fCJD2), two cases with VPSPr (Variably protease-sensitive prionopathy) (VPSPr1 and VPSPr2), CWD and hamster 263K. PrP^Sc captured from sCJD2 and fCJD2 was detectable by 3F4 only on the overexposed film (middle panel shows the overexposed part of the above blot containing samples from sCJD2, fCJD1 and fCJD2). The blots were probed with the 3F4 and 6D11 antibodies and they are a representative of three experiments.