Research Paper Volume 8, Issue 12 pp 3419—3429

Figure 4. Effect of QSOX on PrP^Sc propagation in ScN2a cells. Western blotting of PrP in ScN2a cells in the presence of different amounts of QSOX ranging from 0 to 50 nM for four days. After four days, the cells were lysed and subjected to PK-digestion at 25 µg/ml prior to SDS-PAGE and Western blotting with 6D11. The intensity of PK-resistant PrP^Sc is significantly decreased at 10 nM of QSOX or greater. β-actin was determined to normalize the levels of individual samples examined. The result shown here is a representative of three experiments.