Research Paper Volume 13, Issue 11 pp 15013—15031

Figure 2. Phenotypes of CG9911 mutant flies. (A) Body weight of adult flies (1 day after eclosion) is examined in both CG9911 mutants and w¹¹¹⁸. The numbers of flies are indicated in bracket. (B) Survival curves of CG9911 mutant and w¹¹¹⁸ flies under starvation stimulation. Log-rank (Mantel-Cox) test, p < 0.001. (C) LD staining in CG9911 mutant and w¹¹¹⁸ fat body. BODIPY (green)was used to stain LD. Nuclei were stained by DAPI. Scale bar represents 25 μm. (D) The proportion of stained LD area in the micrograph. Over 15 micrographs in every group were considered for statistics. Data are presented as the means ± s.e.m; ** p<0.01. (E) Relative glyceride levels in whole body of CG9911 mutant and w¹¹¹⁸ flies. Three independent replicates were performed. Glyceride levels were normalized to protein content; * p < 0.05. (F) Real-time PCR of lipogenesis and lipolysis related genes of whole body. All gene detections were subject to three independent replicates. Data are presented as the means ± s.e.m; * p < 0.05, *** p<0.001.