Research Paper Volume 10, Issue 2 pp 278—289
Do senescence markers correlate in vitro and in situ within individual human donors?
- 1 Department of Gerontology and Geriatrics, Leiden University Medical Center, 2300 RC Leiden, the Netherlands
- 2 Unilever Discover, Colworth Science Park, Sharnbrook, Bedfordshire MK44 1LQ, UK
- 3 Netherlands Consortium for Healthy Aging, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
- 4 Department of Molecular Epidemiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
- 5 Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
- 6 Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
- 7 Department of Public Health and Center for Healthy Aging, Faculty of Health and Medical Sciences, 1123 Copenhagen, Denmark
- 8 MOVE Research Institute Amsterdam, Department of Human Movement Sciences, VU University of Amsterdam, 1007 MB Amsterdam, The Netherlands
- 9 Department of Medicine and Aged Care, Royal Melbourne Hospital, Parkville VIC 3050, Australia
Received: September 11, 2016 Accepted: February 23, 2018 Published: February 28, 2018
https://doi.org/10.18632/aging.101389How to Cite
Copyright: Waaijer et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Little is known on how well senescence markers in vitro and in situ correlate within individual donors. We studied correlations between the same and different in vitro markers. Furthermore, we tested correlations between in vitro markers with in situ p16INK4a positivity.
From 100 donors (20-91 years), cultured dermal fibroblasts were assessed for reactive oxygen species (ROS), telomere-associated foci (TAF), p16INK4a and senescence-associated β-gal (SAβ-gal), with/ without 0.6 µM rotenone for 3 days (short-term). In fibroblasts from 40 donors, telomere shortening, ROS and SAβ-gal were additionally assessed, with/ without 20 nM rotenone for 7 weeks (long-term). In skin from 52 donors, the number of p16INK4a positive dermal cells was assessed in situ.
More than half of the correlations of the same senescence markers in vitro between duplicate experiments and between short-term versus long-term experiments were significant. Half of the different senescence marker correlations were significant within the short-term and within the long-term experiments. The different senescence markers in vitro were not significantly correlated intra-individually with in situ p16INK4a positivity. In conclusion, the same and different senescence markers are frequently correlated significantly within and between in vitro experiments, but in vitro senescence markers are not correlated with p16INK4a positivity in situ.