Research Paper Volume 13, Issue 19 pp 23169—23181

LncRNA-SNHG1 promotes macrophage M2-like polarization and contributes to breast cancer growth and metastasis

Shoukai Zong1, , Wei Dai2, , Xiangting Guo3, , Kai Wang4, ,

  • 1 Department of Breast Surgery, The People’s Hospital of Rizhao, Rizhao, Shandong Province, China
  • 2 Department of Breast and Thyroid Surgery, TCM Hospital of Rizhao, Rizhao, Shandong Province, China
  • 3 Department of Rheumatology and Immunology, The People’s Hospital of Rizhao, Rizhao, Shandong Province, China
  • 4 Department of Oncology, The People’s Hospital of Rizhao, Rizhao, Shandong Province, China

Received: January 4, 2021       Accepted: May 14, 2021       Published: October 7, 2021
How to Cite

Copyright: © 2021 Zong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Breast cancer is one of the most common malignant cancers among women. Cancer cells and adjacent cells determine the development of the disease. Tumor associated macrophages (TAMs) are involved in the regulation of different stages of cancer progression. LncRNAs play an important role in tumor growth and metastasis. However, the function of lncRNA in macrophage and tumor cell interaction is poorly described. Here we reported that lncRNA SNHG1 functioned as a modulator of M2 macrophage polarization and regulated tumor growth and angiogenesis. We indicated that knockdown of SNHG1 inhibited M2 macrophage polarization by suppression of STAT6 phosphorylation. SNHG1 silencing significantly alleviated migration of MCF-7 cells and tube formation of Human Umbilical Vein Endothelial Cells (HUVEC). Furthermore, we found that implantation of cell mixture of MCF-7 cells and macrophages promoted tumor growth and angiogenesis. However, knockdown of SNHG1 in macrophages reversed that effect. Collectively, we demonstrated the important role of lncRNA SNHG1 in macrophages and breast cancer cells interaction. We highlight the essential effect of lncRNA in tumor progression and provide a new method for the prevention and treatment of breast tumor metastasis.


TAMs: Tumor associated macrophages; SNHG1: small nucleolar RNA host gene 1; STAT: Signal Transducer and Activator of Transcription; HUVEC: Human Umbilical Vein Endothelial Cells; TME: Tumor microenvironment; LPS: Lipopolysaccharide; IFN-γ: Interferon-γ; TNF-α: tumor necrosis factor-α; VEGF: vascular endothelial growth factor; MMP: matrix metalloproteinase; BMDMs: bone marrow derived macrophages; ATR: all-trans retinoic acid; YM1: chitinase-like 3; ARG1: arginase 1; MRC1: mannose receptor C-type 1; PPAR-γ: peroxisome proliferator activated receptor-γ; CM: condition medium.