Research Paper Volume 14, Issue 23 pp 9758—9772

A pan-cancer analysis of ring finger protein 135 and its relationship to triple-negative breast cancer proliferation and metastasis

Yiqun Yao1, , Guanyu Gong2, , Zijian Guo1, , Dianlong Zhang1, ,

  • 1 Department of Breast and Thyroid Surgery, The Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China
  • 2 Department of Oncology, The Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China

Received: August 25, 2022       Accepted: November 30, 2022       Published: December 10, 2022
How to Cite

Copyright: © 2022 Yao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Ring finger protein 135 (RNF135) is an E3 ubiquitin ligase with RING finger domains that plays a crucial role in the development of several forms of cancer. Neither the expression profile of RNF135 nor its importance in the diagnosis of pan-cancer have been elucidated as of yet. With the aid of The Cancer Genome Atlas and Gene Expression Omnibus, we have fully mapped the expression profiles, prognostic relevance, genetic modification, immune cell infiltration, and tumor heterogeneity of RNF135 in 33 malignant tumors. RNF135 was expressed inconsistently in various cancers, and variations in RNF135 expression predicted survival outcomes for cancer patients. There was a strong correlation between the levels of the RNF135 genetic mutation and some tumor progression. In addition, a strong correlation was seen between RNF135 expression and immune cell infiltration, tumor mutation burden, microsatellite instability, and immunoregulators. In contrast, the correlation between RNF135 expression and triple-negative breast cancer was investigated in this study. RNF135 may boost the proliferation, migration, and invasion of TNBC cells, according to cell experiments. RNF135 might be utilized as a biomarker to anticipate how a tumor will behave and may have a significant role in how TNBC cells grow and migrate, according to the findings of this study.


RNF135: Ring finger proteins 135; TNBC: Triple-Negative Breast Cancer; RIG-I: retinoic acid-inducible gene-I; ICB: immune checkpoint blockade; TME: tumor microenvironment; UCSC: University of California Santa Cruz; TCGA: The Cancer Genome Atlas; GTEx: Genotype-Tissue Expression; TMB: Tumor mutation; MSI: Microsatellite instability; SCID: severely combined immunodeficiency; LGG: Glioma; GBMLGG: Brain Lower Grade Glioma; BRCA: Breast invasive carcinoma; KIPAN: Pan-kidney cohort; OS: overall survival; DFS: disease free survival; CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma; PRAD: Prostate adenocarcinoma; THCA: Thyroid carcinoma; PAAD: Pancreatic adenocarcinoma; HNSC: Head and Neck squamous cell carcinoma; SNV: single nucleotide variants; CNV: copy number variations; TIICs: Tumor infiltrating immune cells.