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• Research Paper Volume 11, Issue 23 pp 11541-11564

  The local translation of K_Na in dendritic projections of auditory neurons and the roles of K_Na in the transition from hidden to overt hearing loss

  Relevance score: 9.103293

  Jeong Han Lee, Mincheol Kang, Seojin Park, Maria C. Perez-Flores, Xiao-Dong Zhang, Wenying Wang, Michael Anne Gratton, Nipavan Chiamvimonvat, Ebenezer N. Yamoah

  Keywords: hearing loss, potassium channels, age-related hearing loss, auditory neurons, axonal protein translation

  Published in Aging on December 8, 2019

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  Local and privileged expression of dendritic proteins allows segregation of distinct functions in a single neuron but may represent one of the underlying mechanisms for early and insidious presentation of sensory neuropathy. Tangible characteristics of early hearing loss (HL) are defined in correlation with nascent hidden hearing loss (HHL) in humans and animal models. Despite the plethora of causes of HL, only two prevailing mechanisms for HHL have been identified, and in both cases, common structural deficits are implicated in inner hair cell synapses, and demyelination of the auditory nerve (AN). We uncovered that Na⁺-activated K⁺ (K_Na) mRNA and channel proteins are distinctly and locally expressed in dendritic projections of primary ANs and genetic deletion of K_Na channels (Kcnt1 and Kcnt2) results in the loss of proper AN synaptic function, characterized as HHL, without structural synaptic alterations. We further demonstrate that the local functional synaptic alterations transition from HHL to increased hearing-threshold, which entails changes in global Ca²⁺ homeostasis, activation of caspases 3/9, impaired regulation of inositol triphosphate receptor 1 (IP₃R1), and apoptosis-mediated neurodegeneration. Thus, the present study demonstrates how local synaptic dysfunction results in an apparent latent pathological phenotype (HHL) and, if undetected, can lead to overt HL. It also highlights, for the first time, that HHL can precede structural synaptic dysfunction and AN demyelination. The stepwise cellular mechanisms from HHL to canonical HL are revealed, providing a platform for intervention to prevent lasting and irreversible age-related hearing loss (ARHL).

  

  sm-FISH and immunocytochemistry localize transcripts and proteins for K_Na1.1 and K_Na1.2 in axons and cell bodies of spiral ganglion neurons (SGNs). Expression of K_Na1-encoding transcripts in the SGNs was examined using smFISH and standard immunocytochemistry in the organ of Corti (OC)/SGN preparations from 1-mo old C57 mice (B–I). (A) Schematic illustration of the inner hair cell (IHC), type I SGN, the peripheral axon, and cell body. The unmyelinated terminal, heminode, and nodes of Ranvier are noted, but not to scale. (B) RNA molecules encoding for K_Na1.1 (Kcnt1), and (C) K_Na1.2 (Kcnt2), were detected as fluorescent spots (purple, arrow) in TUJ1-positive (green) SGN axons, IHCs were labeled with myosin 7A antibody (red), and merged images are shown. Axonal Kcnt1 mRNA were prominent, but only scant Kcnt2 mRNA spots were detected compared to the double knockout (DKO) samples (J). Scale bar = 10 μm (D–E) Images of cochlear sections of 1-mo old mice show that K_Na1.1 (red) protein is expressed in the auditory nerve in D. Consistent with the faint expression of Kcnt2 mRNA in the axons in (E) there was virtually little or no detectable expression of K_Na1.2 in axons of the auditory nerve. Scale bar = 10 μm. (F–G) mRNA spots (purple spots) encoding K_Na1.1 (Kcnt1), and K_Na1.2 (Kcnt2) in the cell bodies of SGNs. Very few spots for Kcnt2 mRNA were detected. Sections were co-labeled with neuronal (TuJ1, green) and nuclei markers (4,6-diamidino-2-phenylindole, DAPI, blue) Scale bar = 5 μm. (H–I) Images of the SGNs show K_Na1.1 (red) protein is expressed in cell bodies of the auditory nerve. In keeping with low levels of expression of mRNA, K_Na1.2 protein expression was faintly positive. The mean number of RNA molecules detected per SGN was calculated as described in the Methods. Kcnt1 levels were higher compared to Kcnt2 in both mRNA and protein levels. (J) (Upper panel). Photomicrograph showing SGN mRNA spots (red spots) encoding Gapdh (data was obtained from DKO tissue). (Lower panel) DKO cochlear section, using kcnt1 probe serving as negative controls. Similar data were obtained using the kcnt2 probe (data not shown). Scale bar = 5 μm. (K) Values of mRNA spots in axons and cell bodies were normalized against Gapdh mRNA spots/100 μm² (11 ± 2 spots (n = 31)) are summarized in the form of bar graphs. The mean (mean ± SD) was (cell body, kcnt1 = 0.38 ±.0.11; kcnt2 = 0.16 ± 0.06; DKO = 0.02 ± 0.04; n = 11 animals; derived from 50 randomly selected cells and evaluated by 5 blinded individuals. The mean (mean ± SD) was (axons, kcnt1 = 0.22 ±.0.07; kcnt2 = 0.08 ± 0.06; DKO = 0.03 ± 0.05; n = 11 animals; derived from 50 randomly selected cells and evaluated by 5 blinded individuals.

  
  
  

  

  K_Na1 DKO mice have reduced and delayed wave I auditory brainstem responses (ABR) but normal absolute thresholds from ages 1 to 3-mo-old in comparison with WT animals, and by 6-mo-old, showed a profound increase in absolute thresholds, relative to the WTs. ABRs were measured in 1-, 3-, 6- and 9-mo old WT and DKO mice. (A–D) Representative ABR traces in response to 80 dB SPL sound clicks are shown for WT (black) and DKO (light grey), at ages 1-, 3-, 6- and 9-mo old as indicated. (E) Average waves I and II amplitudes (input/output, I/O) linear regression slope functions were determined for responses to sound clicks. Wave I, but not wave II, amplitudes were significantly reduced in DKO mice compared to WT mice in response to click sound in 1-mo old. In 3-mo old wave II was significantly different. The mean values for 1-mo old mice for wave I (I/O slope (μV/dB); mean ± SD) were; WT = 0.19 ±.0.04; DKO = 0.10 ± 0.04; n = 11; p < 0.0001. Mean wave II values were; WT = 0.60 ±.0.05; DKO = 0.61 ± 0.08; n = 11; p = 0.7. The mean values for 3-mo-old mice for wave I (I/O slope (μV/dB); mean ± SD) were; WT = 0.20 ±.0.05; DKO = 0.11 ± 0.04; n = 11; p < 0.0001. Mean wave II values were; WT = 0.61 ±.0.07; DKO = 0.50 ± 0.09; n = 11; p = 0.004. (F) Average waves I and II latency (input/output, I/O) functions versus sound clicks. Wave I latency I/O linear regression slopes were significantly different between WT and DKO mice. The mean values for 1-mo old mice for wave I latency (I/O slope (μs/dB); mean ± SD) were; WT = 8.60 ±.0.82; DKO = 11.05 ± 1.57; n = 11; p < 0.0002. Mean wave II values were; WT = 24.40 ±.5.01; DKO = 35.09 ± 4.02; n = 11; p = 0.0001. The mean values for 3-mo old mice for wave I (I/O slope (μV/dB); mean ± SD) were; WT = 10.73 ±.0.73; DKO = 18.93 ± 2.02; n = 11; p < 0.0001. Mean wave II values were; WT = 31.09 ±. 7.11; DKO = 35.80 ± 9.12; n = 11; p = 0.192. Additionally, the I/O slope (μV/dB) of wave I amplitude at 6-mos and 9-mos were; WT = 0.16 ± 0.05; DKO = 0.08 ± 0.06; n = 10; p = 0.005 and (9-mos); WT = 0.14 ± 0.08; DKO = 0.05 ± 0.02; n = 10; p = 0.04. The I/O slope (μs/dB) of wave I latency at 6-mos and 9-mos were; WT = 12.37 ± 0.91; DKO = 20.81 ± 3.11; n = 10; p < 0.0001 and (9-mos); WT = 13.21 ± 1.06; DKO = 22.63 ± 4.11; n = 10; p < 0.0001. (G) Mean absolute ABR thresholds in response to click stimulus were not statistically significantly different between WT and DKO mice in 1-3 mo old but were significantly different in 6- and 9-mo old mice. The mean values for ABR thresholds for 6-mo old were (dB SPL); WT = 24.44 ± 5.27; DKO = 57.22 ± 16.41; n = 9; p = 0.0002. Mean thresholds for or 9-mo old were; WT 46.25 ± 8.76; DKO = 83.13 ± 11.93; n = 8; p < 0.0001.

  
  
  

  

  Changes in pure tone responses. (A–D) Mean absolute ABR thresholds in response to pure tones (4, 8, 16 and 32 kHz) were not statistically significantly different between WT and DKO mice in 1- and 3-mo old, but were significantly different in 6- and 9-mo old mice. Summary data are expressed as (mean ± SD), and statistical comparisons are shown. For 4 KHz, the mean thresholds (in dB SPL) at 6 mos were; WT = 35.00 ± 5.00; DKO = 62.50 ± 12.94; n = 8; p = 0.0001. Mean thresholds at 9 mos were; WT = 45.83 ± 3.76; DKO = 73.33 ± 11.55; n = 8; p = 0.0001. For 8 KHz, the mean thresholds (in dB SPL) at 6 mos were; WT = 46.00 ± 6.52; DKO = 70.83 ± 8.60; n = 8; p = 0.0001. Mean thresholds at 9 mos were; WT = 48.30 ± 4.10; DKO = 78.30 ± 2.89; n = 8; p = 0.0001. For 16 KHz, the mean thresholds (in dB SPL) at 3 mos were; WT = 15.83 ± 4.91; DKO = 28.50 ± 8.83; n = 8; p = 0.0032. Mean thresholds at 6 mos were; WT = 27.50 ± 11.90; DKO = 53.00 ± 2.74; n = 8; p = 0.0001. Mean thresholds at 9 mos were; WT = 25.00 ± 10.49; DKO = 55.00 ± 0.00; n = 8; p = 0.0001. For 32 KHz, the mean thresholds (in dB SPL) at 6 mos were; WT = 42.50 ± 10.40; DKO = 73.84 ± 8.37; n = 8; p = 0.0001. Mean thresholds at 9 mos were; WT = 50.00 ± 4.47; DKO = 80.00 ± 0.00; n = 8; p = 0.0001.

  
  
  

  

  Membrane properties of SGNs from WT and DKO mice. To minimize experimental variabilities, SGN recordings were performed using mice, with recorded ABR. Current-clamp recordings were performed on SGNs isolated from the basal and apical one-third of the cochlea from 1-, 3-, 4-5- (not shown), 6- and 9-mo old WT and DKO mice. (A) Action potentials (AP) evoked from 1-mo-old SGNs isolated from cochlear apical turn from WT (black trace) and DKO (grey trace) mice. Both the resting membrane potential (RMP) and AP amplitude were significantly altered in WT versus DKO SGNs. RMP of apical SGNs; WT, -65 ± 3 mV, n = 31; DKO, -58 ± 2 mV, n = 27; p < 0.001: RMP of basal SGNs; WT, -57 ± 2 mV, n = 25; DKO, -53 ± 4 mV, n = 31; p < 0.001). AP amplitudes were; apical SGN; WT, 80.0 ± 2.7 mV, n = 15; DKO, 72.1 ± 4.8 mV, n = 15; p < 0.001: basal SGN; WT, 83.2 ± 3.4 mV, n = 17; DKO, 72.0 ± 5.1 mV, n = 19; p < 0.001). Dotted black and gray lines indicate 0 mV. (B, C) Examples of slow adapting SGNs isolated from a 1-mo-old basal cochlear turn. For the example shown in (B) typical spike frequency (in Hz) for WT SGNs = 45 ± 7 (n = 17) and DKO = 67 ± 11 (n = 21); p < 0.0001. (D) The dairy plot of the inter-spike interval of slow adapting SGNs from WT and DKO. (E, F) 50 consecutive subthreshold depolarization (0.075 nA current injection) of WT SGNs (E, black traces), and DKO (F, grey traces), demonstrating the extent of membrane jitters. Plotted below is the corresponding standard deviation. (G, H) 30 consecutive suprathreshold depolarization (0.2 nA current injection; interstimulus interval, 2s) of WT SGNs (G, black traces), and DKO (H, grey traces), demonstrating the extent of membrane jitters in evoked APs. Plotted below is the corresponding standard deviation. DKO membrane voltage is pre-disposed to increased membrane jitters. (I, J) APs evoked in SGNs from 9-mo old WT (upper panel) cochlea were generally slower to initiate and larger in amplitude compared to those evoked in SGNs from DKO (lower panel) cochlea. The magnitudes of the injected current are indicated. (K) Across SGNs, the excitability of DKO SGNs has plummeted by several-fold. For the example shown, the spike frequency was reduced by ~7-fold.

  
  
  

  

  Changes of membrane properties of WT versus DKO SGNs isolated from cochlear apex and base at different ages (1- 3- 4-5- 6- and 9-month). (A) The resting membrane potential (RMP) of SGNs was significantly altered at different stages in development. (B) Changes in AP amplitudes of WT and DKO SGNs from apical and basal cochlea are summarized. (C) Amplitude of afterhyperpolarization (AHP) measured and summarized (*p < 0.05; **p < 0.01; n = number of SGNs are indicated). For these data, significant differences between genotypes were determined using the unpaired two-tailed t-test. Other action potential parameters such as latency and duration were measured but not shown (we did not identify any significant changes in action potential latency and duration in WT versus DKO at different stages of development and location in the cochlea).

  
  
  

  

  Reduced Na⁺ current density and available current in K_Na1 DKO SGNs. (A) Whole-cell inward Na⁺ currents were elicited using depolarizing steps from -70 mV to 35 mV (ΔV = 2.5 mV). The currents were normalized to individual membrane capacitance (C_m), were recorded from 1-mo-old mice using apical SGNs from WT (shown with black traces) and DKO (shown with grey traces). Data were generated from 19 SGNs from each experimental group. (B) We observed consistent differences in peak Na⁺ current (I)- voltage (V) relation among WT (●) apical and DKO (●) SGNs. (C) Voltage-dependent inactivation was tested at -10 mV, using pre-pulse voltages ranging from -90 mV to 60 mV (ΔV = 5 mV) (see representative traces, inset). The mean normalized peak recovered-current versus voltage relation for WT (black line and symbol) and DKO (grey line and symbol) SGN from apical third of the cochlea are plotted (n = 9). The half-inactivation potentials (V_1/2) were measured by fitting plots to the Boltzmann function. The V_1/2 for WT was -62.9 ± 2.5 mV and the slope factor k, was, 6.2 ± 0.7 mV (n = 11) and DKO was -66.3 ± 2.7 mV and the slope factor k, was 6.9 ± 0.3 mV (n = 13). (D, E) WT (D) and DKO (E) activation and inactivation curves to illustrate window current and sustained/persistent Na⁺ currents available for activation of K_Na1 current. The activation curves for Na⁺ currents from WT and DKO were fitted with the Boltzmann function. The V_1/2 for activation WT SGN was -40.4 ±3.6 mV, and the slope factor k, was 2.9 ± 0.5 mV (n = 9) and DKO was -45.1 ± 4.8 mV, and the slope factor k, was 3.08 ± 0.8 mV (n = 11).

  
  
  

  

  Reduced Na⁺ current density and available current in K_Na1 DKO SGNs. (A) Whole-cell inward Na⁺ currents were elicited using depolarizing steps from -70 mV to 35 mV (ΔV = 2.5 mV). The currents normalized to individual membrane capacitance (C_m), were recorded from 1-mo-old mice using basal SGNs from WT (shown with black traces) and DKO (shown with grey traces). Data were generated from 15 SGNs of each experimental group. (B) Consistent differences were noted in the peak Na⁺ current (I)- voltage (V) relation among WT (●) apical and DKO (●) SGNs. (C) In WT ~20% of the Na⁺ current is sustained, and in the DKO, ~10% persisted. Voltage-dependent activation and inactivation curves fitted with Boltzmann functions. Half-inactivation potentials (V_1/2) were measured by fitting plots to them. The V_1/2 of the steady-state inactivation for WT was -69.5 ± 3.1 mV and the slope factor k, was, 5.1 ± 0.9 mV (n = 9) and DKO was -66.3 ± 2.7 mV and the slope factor k, was 7.3 ± 0.7 mV (n = 11). The activation curves for Na⁺ currents from WT and DKO were fitted with the Boltzmann function. The V_1/2 for activation WT SGN was -45.1 ± 2.8 mV, and the slope factor k, was 3.1 ± 0.8 mV (n = 9) and DKO was -43.8 ± 2.6 mV, and the slope factor k, was 2.7 ± 0.5 mV (n = 11).

  
  
  

  

  Ca²⁺ transients in 1-mo old SGNs from WT and DKO mice. (A) Representative examples of line-scan images (inset) captured from unstimulated but spontaneous Ca²⁺ oscillations showing Ca²⁺ transients recorded from WT (upper panel, in black) and DKO (lower panel, in gray) SGNs. Sample records show SGN loaded with Fluo 4. (B) Summary data for the fast and slow time constant (τ₁ and τ₂) of the Ca²⁺ transient decay at baseline using two exponential functions. The τ₁ (ms) for WT = 48.4 ± 7.9; DKO = 73.2 ± 6.3; n = 19 (p = 0.019): and τ₂ (ms) for WT = 251.7 ± 24.5; DKO = 373.3 ± 44.2; n = 17 (p = 0.022). (C) Sample records show a region of interest (ROI) of ratiometric Fura 2 assessment of field potential (10V/4Hz) depolarization of WT and DKO SGN (arrows show the time of stimulation). Recovery after field stimulation had ~500-1000s time course (not shown). (D, E) Summary data for the amplitude of the total Ca²⁺ at baseline and after field stimulation in WT and DKO SGNs (1-mo old apical neurons). The F_340/380 for WT before field stimulation = 0.23 ± 0.01 (n = 28); DKO = 0.29 ± 0.03; n = 14 (p = 0.029): and after field stimulation WT = 0.29 ± 0.03 (n = 17); DKO = 0.46 ± 0.06; n = 11 (p = 0.007). Significant differences between genotypes were determined using unpaired two-tailed t-test.

  
  
  

  

  Presynaptic and postsynaptic marker counts are normal in K_Na1 DKO in 1-3-, but not 6-9-mo old mice. Synapses between the spiral ganglion neurons (SGNs) and inner hair cells (IHCs) were quantified at four tonotopic locations (4, 8, 16, and 32 kHz) in the organ of Corti isolated from 1-9-mo old WT and DKO mice. (A–D) There were no obvious differences between WT and DKO mice in the organization of afferent synapses, identified as paired CtBP2 (red) and PSD95-(green) immunopuncta. Images presented as Z-projections were made using stacks of confocal micrographs from the 4, 8, 16, and 32 kHz region as indicated. (E–H) Quantification of the average number of synapses per IHC showed no statistically significant differences between WT (black) and DKO (grey) animals at 1 month of age. However, with increasing age from 3- to 9-mo, statistical differences among the synapse counts appeared and gradually encompassed more tonotopic regions. Statistical differences were observed first at the high-frequency segment of the cochlea. Values (mean ± SEM) are illustrated (p < 0.05, 0.01, 0.001 = *, **, ***). Scale bar = 3 μm.

  
  
  

  

  Myelin density and neurite thickness are unaltered in 1.5-5-old WT and DKO mice. (A) Sections of 1.5- and 5-mo-old WT and DKO spiral ganglion (SG) labeled with Tuj1 (green), and myelin (red) antibodies. Sections were obtained from the basal region of the cochlea. (B) Sections of 1.5 and 5-mo old WT and DKO peripheral neurites of SGN labeled as in (A). (C) Box plot of neurite thickness (diameter) at different age groups. Scale bar = 10 μm.

  
  
  

  

  Age-related degeneration of SGN through apoptosis signal. (A–D) Immunofluorescence detection of cleaved caspase 3 (red; A–B) and caspase 9 (red; C, D) in WT and DKO 5-mo old cochlear sections. SGNs were labeled with neuronal marker TuJ1 (green). The nuclei were stained with DAPI (blue). Scale bar: 20 μm. Increased active caspase 3/9 was seen in DKO cochlear sections. Scale bar = 10 μm. (E) Shown are cochlear sections of in DKO cochleae at difference ages (1-9 mos) assessed for with TUNEL assay (TUNEL-positive in red). Scale bar: 5 μm. (F) Summary histogram showing a significant reduction in SGN size (diameter) between WT and DKO mice at 6-mo old (data from 9 mice each). (G) Age-dependent (1-9 mo) reduction in SGN densities (data from 8-9 mice each). Comparison between WT and DKO mice. (H) Summary data from WT and DKO cochleae at ages indicated, showing increased TUNEL-positive SGNs in 6-9–mo old (data from 8-9 mice each). Significant differences between genotypes were determined using unpaired two-tailed t-test (* p < 0.05, ** < 0.01, *** < 0.001).

  
  
  

  

  Detection of IP3R1, pIP3R1, and its proteolytic fragments in SGNs from WT and DKO cochlea. (A–F) Immunofluorescence detection of the endoplasmic reticulum (ER)-lumen domain of IP3R1(red), phosphorylation site (pIP3R1) (cyan; A–C), N-terminal domain (N-IP3R1) (cyan; C–F). We examined basal cochlear sections at 1.5-mo and 5-mo WT and DKO mice. SGNs were labeled with neuronal marker TuJ1 (blue). The ER was stained with ER-marker (blue). Merged images are shown together with digitally magnified (~3X) images, shown in the last panel. Scale bar 3 μm. For pIP3R1 at 1.5 mos, the percent of SGNs with positive reactivity for WT was 12 ± 3, and DKO was 54 ± 9; (p < 0.0001, data from 7 mice obtained from 25 section/mice). N-IP3R1 at 1.5- and 5-mos, percent cell reactivity for WT = 6 ± 3; DKO = 37 ± 12, and WT = 9 ± 4; DKO = 61 ± 12, respectively (p < 0.0001, data from 6 mice obtained from 25 section/mice). Scale bar = 10 μm.

  
  
  

• Research Paper Volume 10, Issue 7 pp 1640-1648

  Caloric restriction effects on liver mTOR signaling are time-of-day dependent

  Relevance score: 7.834228

  Richa Tulsian, Nikkhil Velingkaar, Roman Kondratov

  Keywords: metabolism, mTOR signaling pathway, aging, glucose, biological clocks, circadian clocks, insulin sensitivity, protein translation

  Published in Aging on July 16, 2018

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  The regulation of mechanistic target of rapamycin (mTOR) signaling contributes to the metabolic effects of a calorie restriction (CR) diet. We assayed the effect of CR on the activity of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) in the liver of mice at six different times across the day. CR effects on mTORC1 and mTORC2 activities were time-of-day dependent. CR induced mTORC1 activity at one time, reduced at two times and has no effect during other times. CR induced mTORC2 activity at one time of the day and has no effects at other times. Circadian clocks are implemented in the regulation of mTOR signaling in mammals and mechanisms of CR. We assayed the effect of CR on mTOR signaling in the liver of mice deficient for circadian transcriptional regulators BMAL1 and CRYs. The CR induced suppression of mTORC1 activity was observed in both clock mutants, while up regulation of mTORC2 was observed in the liver of CRY deficient but not in the liver of BMAL1 deficient mice. Our finding revealed that CR has different time dependent effect on the activity of mTOR complexes 1 and 2 and suggest that circadian clock protein BMAL1 is involved in the up regulation of mTORC2 upon CR in mammals.

  

  CR affects TORC1 activity across the day independently from BMAL1 and CRYs. Activity of mTORC1 in the liver of mice on AL and CR diets was assayed through phosphorylation of ribosomal protein S6 on Ser235/236. Representative WBs and quantification of diurnal rhythms in phosphorylation of S6 in wild type mice (upper panels), Bmal1^-/- mice (middle panels) and Cry1,2^-/- mice (lower panels). (AL) – black diamonds, black solid line, 30% caloric restriction (CR) – black squares and solid black lines. Two-way repeated ANOVA were used for statistical analysis, * - statistically significant difference (p<0.05) between diets (three male mice were used for every time point in both groups). Light and dark bars on the top of the figure represent light and dark phase of the day. ZT0 is the time when light is on and ZT12 is the time when light is off. Food for CR group was provided at ZT14. On the day of tissue collection at ZT14 tissues were harvested before mice received the food.

  
  
  

  

  CR effect on TORC2 activity is time of the day and BMAL1 dependent. Activity of mTORC2 in the liver of mice on AL and CR diets was assayed through phosphorylation of AKT on S473. Representative WBs and quantification of diurnal rhythms in phosphorylation of AKT in wild type mice (upper panels), Bmal1^-/- mice (middle panels) and Cry1,2^-/- mice (lower panels). (AL) – black diamonds, black solid line, 30% caloric restriction (CR) – black squares and dashed black lines. Two-way repeated ANOVA were used for statistical analysis, * - statistically significant difference (p<0.05) between diets (three male mice were used for every time point in both groups). Light and dark bars on the top of the figure represent light and dark phase of the day. ZT0 is the time when light is on and ZT12 is the time when light is off. Food for CR group was provided at ZT14. On the day of tissue collection at ZT14 tissues were harvested before mice received the food.

  
  
  

  

  CR effect on AKT activity is time of the day and BMAL1 dependent. Activity of AKT in the liver of mice on AL and CR diets was assayed through phosphorylation of PRAS on S246. Representative WBs and quantification of diurnal rhythms in phosphorylation of PRAS in wild type mice (upper panels), Bmal1^-/- mice (middle panels) and Cry1,2^-/- mice (lower panels). (AL) – black diamonds, black solid line, 30% caloric restriction (CR) – black squares and dashed black lines. Two-way repeated ANOVA was used for statistical analysis, * - statistically significant difference (p<0.05) between diets (three male mice were used for every time point in both groups). Light and dark bars on the top of the figure represent light and dark phases of the day. ZT0 is the time when light is on and ZT12 is the time when light is off. Food for CR group was provided at ZT14. On the day of tissue collection at ZT14 tissues were harvested before mice receive the food.

  
  
  

• Editorial Volume 10, Issue 3 pp 297-298

  Accurate translation is important for longevity

  Relevance score: 11.696844

  Zhonghe Ke, Andrei Seluanov, Vera Gorbunova

  Keywords: longevity, aging, protein translation, translation fidelity

  Published in Aging on March 12, 2018

  

  Errors in protein translation lead to perturbed proteostasis, protein aggregates and inflammation.