Research Paper Volume 13, Issue 7 pp 10099—10111

Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice

Ci Han1, , Nana Guo2, , Yue Bu2, , Yahui Peng1, , Xueting Li2, , Xiaohui Ma1, , Mengyuan Yang1, , Xiaonan Jia1, , Jin Zhang1, , Xiaowei Liu1, , Kaijiang Yu1, , Changsong Wang1,2, ,

  • 1 Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150001, Heilongjiang, China
  • 2 Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received: January 3, 2021       Accepted: February 18, 2021       Published: March 26, 2021
How to Cite

Copyright: © 2021 Han et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body.

Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection.

Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them.

Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis.


AGI: acute gastrointestinal injury; SCFA: short-chain fatty acid; ICUs: intensive care units; SIRS: systemic inflammatory response syndrome; MODS: multiple organ failure; IL-1: interleukin-1; IL-6: interleukin-6; TNF-α: tumor necrosis factor-α; AQP9: aquaporin Protein-9 Polypeptide; OCLN: occluding; TJ: tight junction; TLR: toll-like receptor; SRB: sulfate-reducing bacteria; ARGs: antibiotic resistance genes; BZA: benzoylamine; PMs: peritoneal macrophages.