Research Paper Volume 15, Issue 6 pp 2082—2096
Single cell sequencing analysis constructed the N7-methylguanosine (m7G)-related prognostic signature in uveal melanoma
- 1 Department of Burn and Plastic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu, China
- 2 Department of Hepatobiliary Surgery, Jiaxing First Hospital, Jiaxing 314001, Zhejiang, China
- 3 Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China
- 4 Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, Jiangsu, China
- 5 Hepatobiliary/Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China
- 6 Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, Jiangsu, China
- 7 College of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China
Received: December 10, 2022 Accepted: March 6, 2023 Published: March 14, 2023
https://doi.org/10.18632/aging.204592How to Cite
Copyright: © 2023 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Uveal melanoma is a highly malignant tumor in the eye. Its recurrence and metastasis are common, and the prognosis is poor.
Methods: The transcriptome data of UVM were downloaded from TCGA database, and the single cell sequencing dataset GSE139829 was downloaded from GEO database. Weighted co-expression network analysis was used to explore the modules associated with m7G. Lasso regression was used to construct M7G-related prognostic signature. Immune infiltration analysis was used to explore the significance of the model in the tumor immune microenvironment. Finally, cell assays were used to explore the function of key genes in the MUM-2B and OCM-1 cell lines of UVM.
Results: The prognostic signature was constructed by Cox regression and Lasso regression. Patients could be divided into high-risk group and low-risk group by this signature, and the high-risk group had worse prognosis (P<0.05). Cell experiments showed that the proliferation, invasion and migration ability of UVM cell lines were significantly decreased after the knockdown of PAG1, a key gene in signature, which proved that PAG1 might be a potential target of UVM.
Conclusions: Our study explored the significance of m7G in UVM, provided biomarkers for its diagnosis and treatment.
Abbreviations
UVM: Uveal melanoma; WGCNA: Weighted co-expression network analysis; m7G: N7-methylguanosine; LASSO: least absolute shrinkage and selection operator; KNN: K-Nearest Neighbor; CSK: C-terminal Src kinase; PAG1: Phosphoprotein associated with glycosphingolipid microdomains 1.